Histology, molecular biology and embryological methods

In situ hybridization of whole-mount larval zebrafish | extends standard embryo in situ protocols for use at metamorphic stages (10–28 dpf); protocol is still under development and is updated periodically   PDF

In situ hybridization on sections of larval zebrafish | detecting mRNA on cryosections of larval zebrafish   PDF

Microinjection of dechorionated embryos | dechorionating and injecting embryos    PDF

Normal table of post-embryonic zebrafish development | staging zebrafish larvae   PDF   WEB

Fish and rotifers

Stock maintenance and breeding | how we maintain zebrafish and other species by selective breeding, natural spawnings and in vitro fertilization   PDF

High density rotifer culture and feeding | growing marine rotifers for young zebrafish larvae at high density   PDF

Fish maintenance | zebrafish feeding, health evaluation, and system checks  PDF

Fish maintenance supervising | overseeing fish,systems, etc.   PDF

Logs | templates for  daily fish and rotifer data.     WEB

Embryo and larva rearing | zebrafish embryo and larval rearing   PDF

Record keeping | stock and tank labeling   PDF

Recipes | foods, water, etc.   PDF

Staging | post-embryonic staging series.   PDF   WEB

Department of Biology

University of Virginia

Physical and Life Sciences Building

Charlottesville VA 22904






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